Comparison of anti-HCV activities of GRFT, CV-N and SVN against JFH-1.
Anti-HCV activities were evaluated by JFH-1 HCVcc full-replication assay, by measuring HCV core protein output in the culture supernatants of Huh7.5.1 cells 72 hr post-infection. The cytotoxicity was evaluated in parallel using WST-8 assay. The HCV output (open circle) and cell viability (closed square) were plotted as percentage (%) relative to control infections without CV-N (A), SVN (B) or GRFT (C). Compounds were added 15 min prior to viral challenge. Results are expressed as mean and standard deviation from triplicate experiments.