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Comparison of DNA measurement using Pico green and qPCR.

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posted on 2009-09-10, 01:25 authored by Angela Cannas, Glendah Kalunga, Clare Green, Ludovica Calvo, Patrick Katemangwe, Klaus Reither, Mark D. Perkins, Leonard Maboko, Michael Hoelscher, Elizabeth A. Talbot, Peter Mwaba, Alimuddin I. Zumla, Enrico Girardi, Jim F. Huggett

The stability of human and total DNA in urine stored at −20 C with 40 mM EDTA and without EDTA shown the same trends (A). Geometric means +/−95% confidence intervals are plotted for each treatment. Measurement with both human qPCR targets correlate with the direct measurement of total nucleic acids using pico-green (B). QPCR using these targets is an appropriate surrogate measure for total DNA and has a greater dynamic range than pico-green for which the lower threshold for accurate measurements is approximately 10 pg/µl.