Comparison of DNA measurement using Pico green and qPCR.
The stability of human and total DNA in urine stored at −20 C with 40 mM EDTA and without EDTA shown the same trends (A). Geometric means +/−95% confidence intervals are plotted for each treatment. Measurement with both human qPCR targets correlate with the direct measurement of total nucleic acids using pico-green (B). QPCR using these targets is an appropriate surrogate measure for total DNA and has a greater dynamic range than pico-green for which the lower threshold for accurate measurements is approximately 10 pg/µl.