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Community response to CO2 exposure over time.

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posted on 26.11.2013, 03:17 by Sergio E. Morales, William E. Holben

Plot of atmospheric temperature, relative humidity, gene abundance and transcript abundance at time of sampling for six functional genes involved in nitrogen (left panel) and carbon (right panel) cycling under simulated CO2 leakage (grey lines) or background (black lines) conditions. Data is presented sequentially by month (N=June, Y=July-Sept) and by hours from start of the experiment. Grey-shaded bars indicate active CO2 flux (injections) for June and July. Values indicate copy numbers per 5 ng of nucleic acid based on averaged measurements of separate triplicate extractions and at least triplicate qPCR reactions for each extraction (n≥9). Error bars are one standard error of the mean (SE). nifH: nitrogenase gene; nosZ: nitrous oxide reductase gene; nirS: nitrite reductase gene; rbcL: ribulose-1,5-bisphosphate carboxylase oxygenase (RuBisCO); mxaF: methanol dehydrogenase; mcrA: methyl-coenzyme M reductase.

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