Combination index (CI) values were calculated for each of the nine dose combinations tested for each cell line and then clustered to generate synergy heatmaps for HER2 positive breast cancer cell lines treated with AKT inhibitors: A. GSK690693; B. GSK2141795.
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posted on 2015-07-16, 03:02 authored by James E. Korkola, Eric A. Collisson, Laura Heiser, Chris Oates, Nora Bayani, Sleiman Itani, Amanda Esch, Wallace Thompson, Obi L. Griffith, Nicholas J. Wang, Wen-Lin Kuo, Brian Cooper, Jessica Billig, Safiyyah Ziyad, Jenny L. Hung, Lakshmi Jakkula, Heidi Feiler, Yiling Lu, Gordon B. Mills, Paul T. Spellman, Claire Tomlin, Sach Mukherjee, Joe W. GrayBlue indicates a significant synergistic CI value (CI<0.8, upper 95% confidence interval is less than 1), white indicates additivity, and red indicates significant antagonism (CI>1.2, lower 95% confidence interval is greater than 1). Gray represents instances where no CI could be calculated. A significant association between synergy and PI3K pathway mutation status was indicated by clustering, which was confirmed by Fisher’s exact test for A and B. Cell lines in green harbor hotspot PIK3CA mutations.
