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Collagen modifying enzymes are transcriptionally regulated by TGFβ3 in sheet forming AF clones.

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posted on 28.01.2016, 12:39 by Guus G. H. van den Akker, Don A. M. Surtel, Andy Cremers, Stephen M. Richardson, Judith A. Hoyland, Lodewijk W. van Rhijn, Jan Willem Voncken, Tim J. M. Welting

Upper panels: expression analysis of P4HA1-3 genes in AF-S and AF-nS clones at t = 0 and t = 7 days of culturing in TGFβ3. Middle panels: expression analysis of PLOD1-3 genes in AF-S and AF-nS clones at t = 0 and t = 7 days of culturing in TGFβ3. Lower panels: expression analysis of genes involved in cleavage of Collagen type I pro-peptides (BMP1, TLL1) and crosslinking of Collagen fibers (LOX) in AF-S and AF-nS clones at t = 0 and t = 7 days of culturing in TGFβ3. Gene expression was normalized to cyclophillin B mRNA levels. Fold induction (t = 7 TGFβ3 / t = 0) was calculated for each clone separately. Mean and standard deviations are depicted for the three clones together per gene. Statistical significance was assessed by Student’s t-test; * p<0.05.

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