Closely related retrotransposons are differentially sensitive to loss of Tex19.1.
(A) Phylogeny of mouse retrotransposon pol and pro proteins. MMERVK10C sequences are highlighted in red. The MMERVK10C sequences lie within a cluster of IAP-type sequences (yellow). (B) Plot showing the likelihood of IAP probes changing expression in the Tex19.1−/− microarray dataset. (C) qRT-PCR for retrotransposons closely related to MMERVK10C in Tex19.1−/− knockout and littermate control testes at 16 dpp. Expression levels for each repetitive element (mean ± standard error for three animals) were normalized to β-Actin and expressed relative to littermate controls. MMERVK10C env.c and IAP primer sets (Figure S2) were used to assess MMERVK10C and IAPEz expression. Asterisk indicates a statistically significant difference (p<0.05).