Chronic Glomerular Disease in PTIP− Kidneys.
A) Coomassie blue staining of SDS/PAGE gels of urine samples from PTIP+ and PTIP− mice at 1 month and 3 months. Mouse albumin (al) is shown as a control. B) Urine albumin to creatinine ratios (ACR) as measured at 1, 3, and 12 months of age in PTIP+ and PTIP− animals. C) Histological sections from kidneys at 3 and 12 months. Representative glomerular sections were stained with Masson's Trichrome (3 and 12 months) or Periodic Acid-Shiff (12 months). Significant matrix deposition was observed in 12 months old PTIP− glomeruli. D) Low power view of a kidney section at 12 months of age shows tubulointerstitial fibrosis, protein filled cysts, and glomerular sclerosis in PTIP− animals.