Chromosome segregation defects in skp1-a7 cells at MI.
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Time-lapse images of WT and skp1-a7 zygotes at MI with indicated fluorescent proteins filmed at 33°C. (A) GFP-Atb2 for microtubules (green) and Htb1-CFP for histone H2B (red). In skp1-a7, the chromosome bridge is seen at 6∼9 min, followed by an appearance of the bent spindle evident at 9 min. The spindle then collapsed and the rebuilt spindle is seen at 12∼15 min. (B) GFP-Atb2 (green) with Mis6-2mCh for kinetochores (red). (C) GFP-Atb2 (green) with Taz1-2mCh for telomeres (red). Bars, 5 µm.