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Chimera C3 is regulated by SlPatA in vivo in S. enterica.

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posted on 11.06.2014, 02:45 by Alex C. Tucker, Jorge C. Escalante-Semerena

Growth behavior of S. enterica in NCE minimal medium supplemented with acetate (10 mM). A. Growth of S. enterica Δacs pat ΔcobB producing H6-SeAcsWT (triangles), H6-SlAcsWT (circles), or H6-Chimera C3 (squares) harboring either a plasmid expressing SlPatAWT (filled shapes) or an empty vector (open shapes). All media was supplemented with 25 µM IPTG. B. Growth of S. enterica Δacs pat ΔcobB (JE9152) producing H6-SlAcsWT harboring a plasmid producing SlPatAWT induced with IPTG concentrations of 25 µM (open circles), 50 µM (light gray), 100 µM (medium gray), 250 µM (dark gray), or 500 µM (black). For reference, half-filled circles denote an equivalent strain producing H6-SlAcsWT harboring an empty vector induced with 500 µM IPTG. C. Growth of S. enterica Δacs pat ΔcobB (JE9152) producing H6-SeAcsWT (triangles), H6-SlAcsWT (circles), H6-Chimera C3 (squares), or empty vector (diamonds) harboring either a plasmid expressing SlPatAWT (filled shapes) or an empty vector (open shapes). All media was supplemented with 5 µM IPTG. D. Growth of S. enterica Δacs pat cobB+ (JE9894) producing H6-SeAcsWT (triangles), H6-SlAcsWT (circles), or H6-Chimera C3 (squares) harboring either a plasmid expressing SlPatAWT (filled shapes) or an empty vector (open shapes). All media was supplemented with 25 µM IPTG. E. Growth of S. enterica Δacs pat cobB+ (JE9894) producing H6-SlAcs-SeAcs chimera C3 harboring a plasmid producing SlPatAWT induced with IPTG concentrations of 10 µM (open triangle), 25 µM (light gray), 500 µM (medium gray), 100 µM (dark gray), or 250 µM (black). For reference, the inverted, filled triangles denote the growth of an equivalent strain producing H6-SlAcs-SeAcs chimera C3 harboring an empty vector (no SlPatAWT) induced with 500 µM IPTG. F. S. enterica Δacs pat cobB+ strains (JE9894) producing H6-SeAcsWT (circles) or H6-SlAcsWT (squares) are shown growing with high induction (250 µM IPTG) of empty vector control (open symbols) or a plasmids expressing SlPatA. F. All S.D. <0.015 absorbance units.

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