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Characterization of the wild-type and fox2Δ strains by electron and immunoelectron microscopy.

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posted on 08.12.2014 by Frédéric Gabriel, Isabelle Accoceberry, Jean-Jacques Bessoule, Bénédicte Salin, Marine Lucas-Guérin, Stephen Manon, Karine Dementhon, Thierry Noël

Glucose-grown cells (A and B) or oleate-induced cells (C to F) of wild-type (A, C, and E) or fox2Δ (B, D, and F) strains were fixed and prepared for electron microscopy (A to D) or immunoelectron microscopy (E and F). In E and F, cryosections were incubated with a polyclonal hen primary antibody directed against C. lusitaniae Fox2p and antibodies were revealed with immunogold particles conjugated to anti-hen antibodies. P, peroxisome, M, mitochondrion, N, nucleus. Bars, 200 nm.

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