Characterization of oligomeric Aβ42 preparation.

(A) Transmission electron micrograph (magnified×32,000) of 100 µM oAβ42. 10 µL of samples were spotted on a glow-discharged, carbon-coated Formavar grid and incubated for 2 min, washed with distilled water, stained with a 1% (w/v) aqueous uranyl acetate solution, and examined using an H-8100 TEM. Scale bar is 100 nm. (B) ThT fluorescence assay of aggregates of Aβ42. a.u., arbitrary unit.