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Characterization of mClavGR2 and its improved variants.

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posted on 11.12.2012 by Ann L. McEvoy, Hiofan Hoi, Mark Bates, Evgenia Platonova, Paula J. Cranfill, Michelle A. Baird, Michael W. Davidson, Helge Ewers, Jan Liphardt, Robert E. Campbell

(A) Absorbance spectra of mMaple (green line), mClavGR2 (red line) and mEos2 (orange line). Absorbance spectra are normalized to absorbance at 280 nm. (B) Photoconversion from the green state (green lines) to the red state (red lines) of mMaple (diamonds), mClavGR2 (triangles), and mEos2 (circles) for pcFP-H2B fusions expressed in HeLa S3 cells. The red-to-green contrast has been calculated at 47 s and 91 s (indicated with vertical dotted lines). At 47 s the contrast is 5.3 for mMaple, 2.1 for mClavGR2, and 1.8 for mEos2. At 91 s the contrast is 10.8 for mMaple, 4.7 for mClavGR2, and 6.9 for mEos2.

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