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Characterization of integration-free PB iPSCs.

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posted on 21.05.2013, 01:30 by Rui-Jun Su, David J. Baylink, Amanda Neises, Jason B. Kiroyan, Xianmei Meng, Kimberly J. Payne, Benjamin Tschudy-Seney, Yuyou Duan, Nancy Appleby, Mary Kearns-Jonker, Daila S. Gridley, Jun Wang, K-H. William Lau, Xiao-Bing Zhang

(A) PB iPSCs show a typical morphology of human pluripotent stem cells. (B) A representative karyogram of an iPSC clone. All analyzed PB iPSC clones showed a normal karyotype. (C) PB iPSCs express pluripotency markers OCT4, SOX2, NANOG and SSEA4. Shown are representative confocal images captured using the Zeiss LSM 710 confocal microscope with a 20× objective. (D) PB iPSCs form teratoma in immunodeficient mice. H & E staining of representative teratoma from PB iPSCs shows derivatives of 3 embryonic germ layers. Cartilage (mesoderm); glands (endoderm) and neurotubules (ectoderm). Images were acquired using the Olympus microscope with a 20× objective.