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Characterization of heparin-activated ATIII.

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posted on 02.11.2012, 02:43 by Mohammed Asmal, James B. Whitney, Corinne Luedemann, Angela Carville, Robert Steen, Norman L. Letvin, Ralf Geiben-Lynn

(A) Sepharcyl S100 ÄKTA FPLC of purified hep-ATIII. A 9–14 ml fraction was separated, termed as hep-ATIII and used for our experiments. Detection at 260 nm for protein detection and 280 nm for heparin detection is shown. (B) Hep-ATIII purity and molecular weight were also determined by SDS-PAGE and silver staining (Bio-Rad kit) of a 15% slab gel. For molecular weight determination a low-range protein molecular weight marker (Bio-Rad) was used. Lane 1∶2 µg hep-ATIII; lane 2: phosphorylase B (97 kDa), serum albumin (69 kDa), carbonic anhydrase (31 kDa), trypsin inhibitor (21.5 kDa), aprotinin (6.5 kDa).

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