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Characterization of SZ-SMA5.

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posted on 2015-10-16, 04:57 authored by Silvina Epsztejn-Litman, Yaara Cohen-Hadad, Shira Aharoni, Gheona Altarescu, Paul Renbaum, Ephrat Levy-Lahad, Oshrat Schonberger, Talia Eldar-Geva, Sharon Zeligson, Rachel Eiges

(A) Expression of undifferentiated cell specific markers by immunostaining for OCT4 (red nuclear staining, merged onto Hoechst (blue)), for the cell surface marker Tra 1–60 (red, merged onto Hoechst (blue)); and (B) for alkaline phosphatase activity (AP). (C) Expression of undifferentiated cell specific markers: OCT4, REX1, NANOG and SOX2 in SZ-SMA5 and a wild-type (WT) HESC control by RT-PCR. GAPDH expression served as a loading control. (D) Teratoma sections stained by H&E from SZ-SMA5 demonstrating multi-cellular structures derived from the three different embryonic germ layers. (E) A representative normal 46(XX) karyotype in SZ-SMA5 as identified by Giemsa staining of 20 different spreads of metaphase chromosomes.