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Characterization of FT-derived, undifferentiated neurospheres by the expression of NPC, neuronal, and glial markers.

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posted on 2013-06-06, 00:50 authored by Ruchira M. Jha, Ryan Chrenek, Laura M. Magnotti, David L. Cardozo

a) Phase microscopy of a single neurosphere derived from a P10 FT culture after 10 days in vitro (DIV) and 1 passage. b) An individual neurosphere derived from a P5 FT culture (5 DIV) stained for the NPC marker Nestin (i, red), DAPI (ii, blue), and merged (iii). The images in (c–f) are co-stained with DAPI (blue). c) The NPC marker Olig2 (red) is expressed in a neurosphere derived from a P7 FT culture (34 DIV). d) Expression of Vimentin (green) in a proportion of cells of an individual neurosphere derived from a P7 FT culture (60 DIV). e) Immunostaining of Sox2 (red) in some cells from a single neurosphere isolated from a P6 FT culture (30 DIV). f) Weak staining of Musashi (red), with some hot-spots, in a neurosphere isolated from a P6 FT culture (30 DIV). g) (i) Sparse expression of β-Tubulin III (Tuj-1) in a proportion of cells of a neurosphere derived from a P7 FT culture (34 DIV). The expression of GFAP in the same neurosphere (g-ii) is restricted to the peripheral cells in vitro. (iii) Merged images of g(i) and (ii). Scale bars: 100 µm.

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