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Characteristics of PV membranous extensions formed by the ΔNCR1 and parental strains.

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posted on 08.12.2011, 01:52 by Bao Lige, Julia D. Romano, Veera Venkata Ratnam Bandaru, Karen Ehrenman, Jelena Levitskaya, Vera Sampels, Norman J. Haughey, Isabelle Coppens

A. Double IFA on HFF infected with either the ΔNCR1 or parental strain 24 h p.i. using anti-GRA3 and anti-GRA7 antibodies revealing more intense fluorescence on extensions from the PV membrane of the TgNCR1-deficient parasites (insets). B. Quantitative analysis of PV membranous extensions for both strains. HFF cells were infected with either strain for 36 h. The samples were fixed and stained using anti-GRA3 antibodies. Serial optical z-sections of about 50 PV randomly selected were obtained from each strain using fluorescent microscopy. The diameter and length of the PV tubules were measured using Volocity software. Values shown are means ± SD of 3 independent infected monolayers for each condition (P values are 0.008 and 0.334 for the diameter and the length, respectively).

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