Changes of sulfate degree before and after HSulf2 digestion of HS oligosaccharides.
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A. HS DP6 sulfated changes for 0 Ac, 1 Ac and 2 Ac series. B. HS DP6 sulfated changes for 0 Ac, 1 Ac and 2 Ac series. Left Y-axis denotes the sulfation degree represented as the average sulfate number per disaccharide. Right Y-axis denotes the decrease in sulfate degree after HSulf2 digestion in percentage. Data significance was calculated by two-tailed Student's t test, with ** denoting p<0.01 and * denoting p<0.05. NS means difference not significant.