Changes in the glycosylation pattern of PrP in CSF of CJD patients.
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A. The pattern of expression of PrP in two representative CSF samples (from one healthy control individual and one CJD patient) is compared to the PrP pattern observed in control brain tissue using Western blot analysis. B. CSF samples from one healthy control individual, one CJD patient and one healthy control brain tissue were treated with PNGase F and then analyzed by Western blot. Data represent the analysis of three control and five individual CJD samples. C. and D. CSF samples from one healthy control individual and one CJD patient were treated with various concentrations of PK or thermolysin (Ther), respectively, followed by Western blot analysis. Data represent the analysis of three control and five individual CJD samples.