Changes in p47 and ROS generation after transfection of Ad-GFP or Ad-XBP1S.
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posted on 2013-02-19, 15:20 authored by Decui Shao, Jia Liu, Jun Ni, Zhen Wang, Yang Shen, Li Zhou, Yu Huang, Jun Wang, Hong Xue, Wei Zhang, Limin LuA: Western blot analysis on p47phox levels in the cultured MCs before and after Ad-XBP1S transfection for 48 h (mean ± SEM, n = 5). B: Observation of ROS generation by DHE fluorescent probe assay in the cultured MCs before and after Ad-XBP1S transfection for 48 h (mean ± SEM, n = 8). *p<0.05 compared to NG; #p<0.05 compared with HG; ##p<0.01 compared to HG. C: Western blot analysis on collagen IV and fibronectin levels after transfection of Ad-XBP1S or Ad-XBP1S+xanthine and xanthine oxidase (X⋅XO) for 48 h in the presence of HG. **p<0.01 compared to Ad-GFP; #p<0.05 compared with Ad-XBP1S (mean ± SEM, n = 5).
