Changes in LSD1 activity affect cell proliferation in the A549 and H460 cell lines, as detected by immunofluorescence.
Images are shown at ×20 magnification. Green, red and blue fluorescence represent LSD1, BrdU and Topro-3, respectively. The proliferation significantly increased in the A549 cells after transfection of the Flag-LSD1 plasmid. However, proliferation decreased in the H460 cells after transfection of the LSD1 siRNA plasmid. Pargyline treatment reduced proliferation in both cell lines. Topro-3 staining shows nuclear localization, and BrdU staining shows the proliferating cells.