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Change of Sirt1 and AMPK pathway and GLP-1R expression by exendin-4 in mouse livers.

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posted on 20.02.2013, 08:51 by Jinmi Lee, Seok-Woo Hong, Seoung Wan Chae, Dong Hoon Kim, Ji Hun Choi, Ji Cheol Bae, Se Eun Park, Eun-Jung Rhee, Cheol-Young Park, Ki-Won Oh, Sung-Woo Park, Sun-Woo Kim, Won-Young Lee

Total RNA and protein were extracted from liver tissue and (A) Nampt, Sirt1, Lkb1, AMPKα1 and AMPKα1 mRNA and (B) GLP-1R, Sirt1, phospho-AMPKα, AMPK and β-actin protein expression were measured by quantitative real-time RT-PCR and western blot, respectively. Data were normalized to the β-actin of each sample. All values are expressed as the mean ± SE for n = 5–7 mice. * p<0.05, ** p<0.01 compared with a control and # p<0.05, ## p<0.01 compared with a HF.

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