Change of Sirt1 and AMPK pathway and GLP-1R expression by exendin-4 in mouse livers.
Total RNA and protein were extracted from liver tissue and (A) Nampt, Sirt1, Lkb1, AMPKα1 and AMPKα1 mRNA and (B) GLP-1R, Sirt1, phospho-AMPKα, AMPK and β-actin protein expression were measured by quantitative real-time RT-PCR and western blot, respectively. Data were normalized to the β-actin of each sample. All values are expressed as the mean ± SE for n = 5–7 mice. * p<0.05, ** p<0.01 compared with a control and # p<0.05, ## p<0.01 compared with a HF.