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Cellular localization of GFP-tagged ISCL.

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posted on 2009-12-11, 00:55 authored by Ou Zhang, Mattie C. Wilson, Wei Xu, Fong-Fu Hsu, John Turk, F. Matthew Kuhlmann, Yingwei Wang, Lynn Soong, Phillip Key, Stephen M. Beverley, Kai Zhang

(AE) Log phase WT [pXG-GFP-ISCL] parasites were stained with MitoTracker Red580 and subjected to fluorescence microscopy. (A) DIC; (B) Hoechst staining; (C) MitoTracker staining; (D) GFP fluorescence; (E) Merge of C and D. (F) GFP-ISCL is functional. Whole cell lysates from iscl or iscl transfected with pXG-GFP-ISCL were tested for neutral SMase activity as described and a representative TLC image is shown. Positive control (+): 0.1 unit of B. cereus SMase; negative control (−): boiled WT lysate.


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