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Cell morphology, actin, myosin, and phosphorylated-myosin organization following myosin inhibition.

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posted on 2009-09-16, 01:03 authored by Christopher M. Hale, Sean X. Sun, Denis Wirtz

A–C. Typical morphology, actin filament organization (red), distribution of myosin heavy chain IIA (green), and nuclear DNA (blue) in control cells (A), cells treated with 25 µM myosin II inhibitor blebbistatin (B), and cells treated with 20 µM myosin light chain kinase inhibitor ML-7 (C). D–F. Typical morphology, actin filament organization (red), distribution of phosphorylated myosin light chain 2 (green), and nuclear DNA (blue) in control cells (D), cells treated with 25 µM myosin II inhibitor blebbistatin (E), and cells treated with 20 µM myosin light chain kinase inhibitor ML-7 (F). Scale bars, 20 µm.

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