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Calnexin promotes growth in cation-depleted medium.

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posted on 07.12.2011, 01:52 by Margaret V. Powers-Fletcher, Kalyani Jambunathan, Jordan L. Brewer, Karthik Krishnan, Xizhi Feng, Amit K. Galande, David S. Askew

Equal numbers of conidia from the indicated strains were inoculated onto glass coverslips in liquid AMM containing 1 mM EDTA (AMM-EDTA) and incubated at 37°C. (A) Impaired germination of ΔclxA conidia in EDTA: coverslips were removed after 2 and 6 days incubation and fungal morphology was photographed by differential interference contrast microscopy. (B) Abnormal morphology of the ΔclxA mutant after prolonged incubation in EDTA: A high power image of the abnormally swollen conidia and hyphae of the ΔclxA mutant after 6 days of incubation in AMM-EDTA at 37°C is shown. (C) Supplementation with zinc rescues the growth of ΔclxA in AMM-EDTA: The ΔclxA conidia were inoculated into AMM-EDTA medium supplemented with 500 µM ZnS04 and cultured for 2 days at 37°C.

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