Calnexin promotes growth in cation-depleted medium.
Equal numbers of conidia from the indicated strains were inoculated onto glass coverslips in liquid AMM containing 1 mM EDTA (AMM-EDTA) and incubated at 37°C. (A) Impaired germination of ΔclxA conidia in EDTA: coverslips were removed after 2 and 6 days incubation and fungal morphology was photographed by differential interference contrast microscopy. (B) Abnormal morphology of the ΔclxA mutant after prolonged incubation in EDTA: A high power image of the abnormally swollen conidia and hyphae of the ΔclxA mutant after 6 days of incubation in AMM-EDTA at 37°C is shown. (C) Supplementation with zinc rescues the growth of ΔclxA in AMM-EDTA: The ΔclxA conidia were inoculated into AMM-EDTA medium supplemented with 500 µM ZnS04 and cultured for 2 days at 37°C.