CYP3A4 gene expression regulation by calcitriol and astemizole in the hepatoma cell line HuH-7.
Cells were incubated in the presence of increasing concentrations of calcitriol (panel A) or astemizole (panel B) during 24 hours. Afterwards RNA was extracted for real time PCR analysis. Combined effect of both astemizole (3 µM) and calcitriol (10 nM) is shown in panel C. Results were normalized against GAPDH mRNA expression and in all cases vehicle values were set to one. In the box below the graphic: C = Calcitriol, A = Astemizole. N = 3, *P<0.05 vs control. **P<0.05 vs each compound alone.