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CK-B−/− astrocytes spread slower than WT astrocytes.

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posted on 31.03.2009 by Jan W. P. Kuiper, Remco van Horssen, Frank Oerlemans, Wilma Peters, Michiel M. T. van Dommelen, Mariska M. te Lindert, Timo L. M. ten Hagen, Edwin Janssen, Jack A. M. Fransen, Bé Wieringa

(A) Astrocytes derived from WT and CK-B(−/−) mice were compared in quantitative cell spreading assays. Cells were seeded on FN and 30 min after seeding the cells were fixed and stained for F-actin. Bar, 100 µm. (B) Quantification of astrocyte spreading on FN represented as a percentage of the control (see materials and methods for details), ** p<0.01. (C) WT astrocytes spreading on FN co-stained for CK-B and F-actin. CK-B is distributed throughout the cytoplasm and accumulation is seen at membrane ruffles. Co-localization with cortical F-actin is shown in the merged image. Bar, 10 µm. (D) Quantitative cell spreading assay using WT and CK-B−/− astrocytes. Cells were seeded on Laminin (Lam) and 30 min after seeding the cells were fixed and stained for F-actin. Bar, 100 µm. (E) Quantification of astrocyte spreading on Lam showing decreased spreading capacities of CK-B−/− cells, ** p<0.01. (F) Astrocytes spreading on Lam co-stained for CK-B and F-actin. CK-B accumulation is seen at membrane ruffles. Co-localization with cortical F-actin is shown in the merged image. Bar, 10 µm.

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