CIB1 association with macropinocytic marker dextran early during de novo KSHV infection in endothelial cells.

HMVEC-d cells were incubated with medium containing Texas Red labeled dextran (A) or Texas Red labeled transferrin (B) alone (no virus, Uninfected) or with KSHV (20 DNA copies/cell) and Texas Red labeled dextran or Texas Red labeled transferrin at 37°C for the indicated time points. Cells were fixed and processed for immunofluorescence analysis using mouse anti-CIB1 antibodies and co-stained with DAPI. Boxed areas in the merged panel are enlarged in the rightmost panel and arrows indicate colocalization of molecules. Representative deconvoluted immunofluorescence images are shown.