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CGI-58 affect C20PUFA abundance in the lipid droplet membrane.

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posted on 17.06.2015, 03:24 authored by Meng Xie, Richard Roy

(A)-(D) Loss of cgi-58 in AMPK mutant (A and B) and control daf-2 (C and D) dauer larvae led to a lipid droplet-specific reduction of C20PUFAs. LD = lipid droplets. Cyto = cytoplasm. Purified lipid droplets and remaining cytoplasm were subjected to fatty acid methyl ester (FAME) extraction followed by GC/MS analysis. Short/branched fatty acids are the combined total percentage of C14:0, C15ISO, C16ISO, C16:0, C17ISO, C16:1n7 and C17Δ. C18 fatty acids are the combined total percentage of C18:0, C18:1n11, C18:1n10, C18:1n9, C18:1n7, C18:2n6 and C18:3n6. C20PUFAs are total percentage of C20:3n6, C20:4n6, C20:4n3 and C20:5n3. ** indicates statistical significance (P<0.01) and error bars indicate SD of three independent experiments in (A)-(I). (E)-(F) The amount of all major C20PUFAs were decreased in the lipid droplets of daf-2; aak(0); cgi-58 (E) and daf-2; cgi-58 (F) dauer larvae. (G) Long chain fatty acids were decreased in CGI-58 MEF mutants. Total cell lysates were subjected to FAME extraction followed by GC/MS analysis. Long chain fatty acids include fatty acids with a carbon chain longer than 20 and certain aromatic fatty acids. (H) C20PUFAs were lost from the phospholipid portion of the lipid droplets of daf-2; aak(0); cgi-58 dauer larvae. PL = phospholipid. (I) Some C18 fatty acids were completely absent in the triglyceride portion of the lipid droplets of daf-2; aak(0); cgi-58 dauer larvae. TG = triglyceride.

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