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CDK11 depletion reduces protein expression of CDK9 and HEXIM1 and Tat transactivation of HIV-1 provirus.

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posted on 05.09.2008 by Wendong Yu, Rajesh Ramakrishnan, Yan Wang, Karen Chiang, Tzu-Ling Sung, Andrew P. Rice

A. HeLa cells were transfected with siRNAs against CDK11 siRNA or control siRNAs. Cells were harvested 72 h post-transfection and cell lysates were analyzed for expression of CDK11, CDK9, HEXIM1, and CDK8 by immunoblots. B. HeLa cells were infected with a lentiviral DHFR-Renilla Luciferase virus with GFP marker. Cells sorted for GFP were then infected with either HIV-1 NL4-3-firefly Luciferase virus (wild type) or with HIV-1 NL4-3 firefly Luciferase virus encoding a mutant Tat (Tat−) for 48 h. Cultures were transfected with the indicated siRNAs. Cell extracts were prepared 48 h post-transfection and assayed for Luciferase expression. Results are presented as the ratio of firefly to Renilla Luciferase expression.

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