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CD98 regulates serum dependent cell proliferation by increasing Erk and p38 MAPK signaling.

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posted on 2013-02-20, 03:11 authored by Nada Bulus, Chloe Feral, Ambra Pozzi, Roy Zent

(A, C) CD98hc expression increases MAPK, Akt and mTor signaling in response to serum. Subconfluent cells were serum deprived for 24 hours then treated with complete medium. Lysates were prepared at the time points indicated and analyzed by western blot with the antibodies indicated. (B, D) MAPK regulates CD98hc-dependent IMCD cell proliferation. Cells seeded onto 96-well plates were serum deprived for 24 hours, pretreated with inhibitors prior to addition of 3H-Thymidine in complete medium as described in Experimental Procedures. U+SB denotes concomitant treatment with the MEK inhibitor U0126 (U) and the p38 MAPK inhibitor SB203580 (SB). (*) indicates statistically significant differences (p<0.05) between treated and untreated cells. (**) denotes statistically significant differences between U+SB versus either inhibitor alone.

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