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CD4 SP thymocytes, but not Treg cells, store and mobilize pCD40L.

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posted on 21.02.2012, 02:25 by Yoshinobu Koguchi, Abigail C. Buenafe, Timothy J. Thauland, Jennifer L. Gardell, Elizabeth R. Bivins-Smith, David B. Jacoby, Mark K. Slifka, David C. Parker

A, Gating strategy for thymic nTreg cells and CD4 SP thymocytes. B, Generation of in vivo iTreg cells. DO11.10 CD4+ T cells were recovered from OVA- or BSA-fed recipient mice on day 6. C, Gating strategy for in vivo iTreg cells and effector CD4+ T cells. A BSA-fed mouse is shown as a negative control. D, thymic nTreg cells; E, CD4 SP thymocytes; F, in vivo iTreg cells; and G, effector CD4+ T cells, are analyzed by the mobilization assay and intracellular staining for pCD40L and CTLA-4. Data are representative of three (D and E) or two (F and G) independent experiments.

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