CD138+ MMC/OSB N-cadherin mediated interactions.
Data presented is the response of patient sample #4 and it is representative of repeated experiments conducted using a total of 2 different patient samples (Table 1). (a-b) Bright field image and merged florescent image showing that CD138+ MMC (red, CD138 PE labeled) did not adhere to N-cadherin knockdown (green, GFPhigh) OSB. (c-d) Bright field image and merged florescent image of CD138+ MMC seeded onto mock-transduced OSB (green), showing that CD138+ MMC adhered to GFP+ mock-transduced cells. (e) Calculated retention ratio of CD138+ MMC seeded onto mock-transduced or N-cadherin knockdown OSB. After a 72 h coculture period, the retention ratio of CD138+ MMC was found to be significantly decreased when BMMC were seeded onto N-cadherin knockdown vs. mock-transduced OSB. * p < 0.05.