Bioluminescence images of Ins1-luc BAC transgenic mice fed a regular (RD) or a high-fat diet (HFD).
(A) Representative images of mice fed a RD or an HFD for 12 weeks beginning at 6 weeks of age. (B) Quantification of bioluminescence intensity (RD: n = 4; HFD: n = 6). *P = 0.019. (C) Computed tomography (CT)-based body composition analysis. Representative CT images of mice treated with a RD or an HFD for 8 weeks. Purple, yellow, and blue areas represent visceral fat, subcutaneous fat, and lean mass, respectively. (D) Body fat percentage of mice treated with a RD (n = 4) or an HFD (n = 5) for 8 weeks. (E) Fasting blood glucose levels in the RD- (n = 4) or HFD- (n = 5) treated groups of mice for 8 weeks. (F) β-cell mass in the RD- (n = 4) and in the STZ- (n = 5) treated group. P = 0.49 (G) Immunohistochemistry for anti-insulin (Ins) and anti-luciferae (Luc) antibodies with diamidino-2-phenylindole (DAPI) for nuclear staining in the islets of RD- and HFD-fed Ins1-luc BAC transgenic mice Scale bars: 50 µm.