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Biochemical and neuropathological analysis of mice inoculated with in vitro-generated PrPSc molecules.

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posted on 14.07.2011, 02:09 by Michael B. Miller, James C. Geoghegan, Surachai Supattapone

Brains were dissected from wild-type mice showing terminal scrapie signs (PrPSc and ΔC-PBD PrPSc inocula) or similarly aged mice not displaying scrapie signs (mock-propagated, ΔN-PBD PrPSc, and ΔΔ-PBD PrPSc inocula). (A) Equivalent amounts of 10% brain homogenate were treated with buffer (−PK) or 25 µg/mL proteinase K (+PK to show PrPSc) and detected by anti-PrP (6D11) immunoblot. A greater exposure of the same immunoblot is displayed below, to illustrate samples containing low amounts of PrPSc. (B) Neuropathology of cerebellum and hippocampus. Brain sections were stained with hematoxylin and eosin (H&E). The black bar denotes 100 µm.

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