Binding of HES1 to TRCSM promoter sequences.
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(A) Position of HES1 binding sites within the promoter regions of Plcβ2 and Ip3r3 are indicated by green squares. Arrowheads indicate primer pairs used for ChIP assays. Primer pairs p1, ip1, and ip2 amplified the DNA fragment that included HES1 binding sites, while fragments amplified by p2C and ip3C primer sets did not contain the HES1 binding sequence. (B) ChIP results using P19 embryonal carcinoma cells as chromatin substrate. HES1 antibody efficiently precipitated sequences containing Plcβ2 and Ip3r3 promoter HES1 binding sites.