Binding assay of histones and heparin.
Whole blood was collected and centrifuged for plasma 12(0.1 mol/l, 2 µl g−1). The plasma collected from the injury group was incubated with heparin-agarose solution or agarose solution at 37°C for 30 minutes. After incubation the plasma was separated from the heparin-agarose or agarose by centrifugation and measured with an H4 ELISA kit. *p<0.05 vs. the control group, **p<0.01 vs. the control group; #p<0.05 vs. the injury group, ##p<0.01 vs. the injury group.