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Beads improve the amplification efficiency of SSLOW.

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posted on 2011-02-10, 02:36 authored by Nuria Gonzalez-Montalban, Natallia Makarava, Valeriy G. Ostapchenko, Regina Savtchenk, Irina Alexeeva, Robert G. Rohwer, Ilia V. Baskakov

SSLOW scrapie brain material was diluted 103-fold (lanes 1–8) or 104-fold (lanes 9–15) into 10% NBH, subjected to serial PMCA in the absence or presence of 3 small beads, as indicated, and digested with PK. Each PMCA round consisted of 48 cycles; the material amplified in each round was diluted 10-fold into 10% NBH for the next PMCA round. Undigested 10% NBH (lane 1) loaded at 1/10th the amount of the digested samples is provided as a reference.

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