Bacterial colonization, colonic mucosa inflammation and translocation in CEABAC10 mice infected with LF82ΔfimH/fimH_LF82 or LF82ΔfimH/fimH_K12 mutants.

(A) Quantification of LF82-ΔfimH/fimH_LF82 (black square) or LF82-ΔfimH/fimH_K12 (white square) bacteria in the feces of CEABAC10 mice receiving 0.25% DSS in drinking water after oral infection with 10⁹ bacteria on day 0. (B) Quantification of colonic mucosal-associated LF82-ΔfimH/fimH_LF82 or LF82-ΔfimH/fimH_K12 bacteria on day of sacrifice. (C) Quantification of bacteria on day of sacrifice in the liver and spleen. (D) DAI was performed for CEABAC10 transgenic mice infected with (black square) LF82ΔfimH/fimH_LF82 or with (white square) LF82ΔfimH/fimH_K12. (E) Il-1β secretion by colonic mucosa. (F) Histopathological scoring for several parameters of colonic inflammation was performed for CEABAC10 transgenic mice infected with (black square) LF82ΔfimH/fimH_LF82 or with (white square) LF82ΔfimH/fimH_K12. * P<0.05; ** P<0.01; and *** P<0.001.