BRG1 and E2F6 functions in a complex with other proteins.

A) DP1, previously shown to interact with E2F6, is also in a complex together with BRG 1. Plasmid constructs expressing DP1, E2F6, and BRG1 were contransfected into 293 cells. Immunoprecipitation experiments were carried out in lysis buffer with a monoclonal antibody recognizing DP1. Immunoprecipitates were resolved on SDS PAGE and Western blotted for E2F6, BRG1 and DP1. B) BAF155, previously shown to interact with BRG1, is also in a complex together with E2F6. Plasmid constructs expressing BAF155, E2F6, and BRG1 were contransfected into 293 cells. Immunoprecipitation experiments were carried out in lysis buffer with a monoclonal antibody recognizing BAF155. Immunoprecipitates were resolved on SDS PAGE and Western blotted for E2F6, BRG1 and BAF155. C) E2F6 binds to BAF180. Plasmids expressing HA-tagged E2F6 or Flag-tagged BAF180 were co-transfected into 293T cells. Cell lysates were immunoprecipitated with polyclonal antibodies recognizing E2F6 or BAF180. Immunoprecipitates were than resolved on SDS PAGE and Western blotted with monoclonal antibodies recognizing HA or Flag epitope tags.