BCG-pAg85A combination induces Ag85A-specific IgG antibodies.
A. Ag85A-specific antibodies as detected at day -1 before BCG priming and at days 20, 41, 63 and 118 of the vaccination protocol. Mean O.D. values (490/620nm) of individual sera diluted 1:200 are shown for group 1 (black symbols), group 2 (blue symbols) and group 3 (green symbols). B. IgG1:IgG2 ratio of Ag85A specific antibodies measured at day 63 of vaccination on sera diluted 1:50.