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Axonal terminal degeneration in the striatum following intrastriatal LPS.

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posted on 08.05.2009, 02:37 by Dong-Young Choi, Mei Liu, Randy L. Hunter, Wayne A. Cass, Jignesh D. Pandya, Patrick G. Sullivan, Eun-Joo Shin, Hyoung-Chun Kim, Don M. Gash, Guoying Bing

(A) Silver staining reveals that there is no silver-positive stained fibers in the vehicle treated striatum while an abundance of silver grain-deposits are observed in the LPS injected striatum, suggesting the degeneration of axonal fibers (arrows). Scale bar: 20 µm. (B) Immunostaining for DARPP-32 shows that the GABAergic neurons are intact following LPS injections. Western blot for DARPP-32 and its quantification indicate that there is no significant alteration in the expression of DARPP-32 after LPS challenge. Scale bar: 200 µm. (C) HPLC analysis shows that intrastriatal LPS injection depletes 58% of the striatal dopamine relative to control at four weeks. The DOPAC level is not affected; however, HVA is significantly increased. The turnover ratios of DOPAC/dopamine and HVA/dopamine are dramatically increased (n = 7/group; ** p<0.01, *** p<0.001).

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