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AvCystatin stimulation induces transient phosphorylation of p38 and ERK.

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posted on 2011-01-06, 02:23 authored by Christian Klotz, Thomas Ziegler, Ana Sofia Figueiredo, Sebastian Rausch, Matthew R. Hepworth, Nadja Obsivac, Christine Sers, Roland Lang, Peter Hammerstein, Richard Lucius, Susanne Hartmann

Thioglycollate elicited peritoneal macrophages from BALB/c mice were stimulated with 0,5 µM AvCystatin or the same amount of denaturated AvCystatin as a control. After the indicated time points total cell extracts were isolated and analyzed by western blot with antibodies against phospho-p38, total-p38, phospho-ERK, total-ERK, phospho-AKT, total-AKT and beta-actin. (A, C, E) Representative blots are shown. (B, D, F) Densitometric analysis (mean ± SD, n = 3–4) of phospho-p38, phospho-ERK and phospho-AKT normalized to the endogenous control are expressed as fold induction relative to the medium control.

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