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Attachment and infection in CHO parental cells and GAG-negative pgsA-745 cells.

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posted on 04.07.2013, 01:55 by Linda Cruz, Craig Meyers

CHO Par and pgsA-745 cells were plated 2 days prior to infection by A) HPV16 and HPV18 at an MOI of 10 or attachment by B) HPV16 and HPV18, and D) HPV31 and HPV45. Infections were analyzed by RT-qPCR measuring the relative amount of E1^E4 transcript two days post-infection normalizing to infection by the CHO Par cells. Analysis of the number of particles was done by SYBG q-PCR after incubating for 2 hours at 4°C, normalizing attachment to the CHO Par cells.