At least 5 β-thymosins are expressed in P. leniusculus.

A) Putative exon structure of a β-thymosin gene in P. leniusculus determined by sequencing five different spliced transcripts. B) Protein structure determined for Pl-β-thymosin1–5. The typical Tβ domains (pfam 01290) are encoded for by different exons (as indicated by color that reflects the encoding exons in A) and the different actin binding motifs are indicated by triangles (LKKT) and arrows (LKHA). C) Sequence alignments of Pl-β-thymosins with human Tβ4 (accession no. NP_066932.1). Consensus sequences are highlighted with green background. D) Tissue distribution of different Pl-β-thymosins in various tissues of P. leniusculus detected by RT-PCR. The mRNA expression of 40S ribosomal protein is shown as an internal control.