Association mapping demonstrates that a single dominant locus controls resistance to B. malayi in a Kenyan population of Ae. aegypti.
A) Mapping with an additive model of inheritance produces a small number of significant associations on chromosome 1 and in regions unassigned to chromosomes. B) Including the most significant marker in panel A as a covariate in the analysis results in no significant associations, suggesting that a single locus controls infection. C) A dominance model of inheritance produces highly significant associations, whereas D) a recessive model does not. Red-dotted line shows genome-wide significance at P<0.01; black-dotted line shows significance at P<0.05. The x-axis represents a physical map (bp) made by arranging scaffolds along the genetic map , with scaffolds mapping to the same genetic map position being ordered randomly. To correct genome misassemblies, scaffolds that contain any gene with at least 10 segregating sites and an FST greater than 0.1 in the RNA-seq data were moved to the unassembled region. In the dominance model, 13 sites on 4 scaffolds map to 0 cM and a single site maps to 12 cM.