Assessment of lipid droplet content in AF-HSCs and GFP-HSCs after overnight culture.
figureposted on 2011-09-16, 01:17 authored by Diana N. D'Ambrosio, José L. Walewski, Robin D. Clugston, Paul D. Berk, Richard A. Rippe, William S. Blaner
AF-HSCs and GFP-HSCs were isolated by FACS and incubated at 37°C overnight on glass bottom dishes. Phase contrast and fluorescent images were captured at 60× magnification for both AF-HSCs (A) and GFP-HSCs (B). Scale bars represent 30 µm. Average number of lipid droplets (C), average diameter (µm) of lipid droplets (D) and total lipid droplet volume (µm3) (E) per HSC are shown. (F) Protein levels of ADRP in AF-HSCs and GFP-HSCs. Experiment was repeated with similar results.