Assessment of lipid droplet content in AF-HSCs and GFP-HSCs after overnight culture.

AF-HSCs and GFP-HSCs were isolated by FACS and incubated at 37°C overnight on glass bottom dishes. Phase contrast and fluorescent images were captured at 60× magnification for both AF-HSCs (A) and GFP-HSCs (B). Scale bars represent 30 µm. Average number of lipid droplets (C), average diameter (µm) of lipid droplets (D) and total lipid droplet volume (µm³) (E) per HSC are shown. (F) Protein levels of ADRP in AF-HSCs and GFP-HSCs. Experiment was repeated with similar results.