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Assembly of three DNA fragments using NE-LIC coupled with in vivo circularization.

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posted on 06.03.2013, 14:51 authored by Rui-Yan Wang, Zhen-Yu Shi, Ying-Ying Guo, Jin-Chun Chen, Guo-Qiang Chen

(A) Schematic diagram of the three DNA fragments assembly. (B) Three fragments assembly using both 3′-end (5′-end digestion using Nb.BbvCI) (left) and 5′-end single-stranded (3′-end digestion using Nt.BspQI) (right) annealing. DNA gel electrophoresis verified the assembly results (B-middle).