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Arsenic and temsirolimus combination treatment results in decreased phospho-S6 and ERK activation.

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posted on 2013-12-31, 05:43 authored by Cynthia Guilbert, Matthew G. Annis, Zhifeng Dong, Peter M. Siegel, Wilson H. Miller Jr, Koren K. Mann

MDA-MB-468, MCF-7, SkBr3, and T47D cell lines were exposed to vehicle control (Vh; 5.3 x 10-6 N NaOH and 2.5 x 10-5 % EtOH in PBS), 1-2 µM ATO, 0.5-5 ng/ml temsirolimus and the combinations for 24 hours. Whole cell extracts were used in immunoblotting experiments for phospho-S6, S6, phospho-ERK, ERK, and β-actin. Immunoblots shown are representative of experiments performed at least 3 times.