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Array Comparative Genomic Hybridization (a-CGH) of each adaptive clone versus their common ancestor, JA122.

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posted on 06.11.2009, 01:55 by Margie A. Kinnersley, William E. Holben, Frank Rosenzweig

CV103 has sustained an approximately 29 Kb deletion relative to JA122 comprising a total of 27 genes of either unknown function or involved in transcription, arginine biosynthesis, anaerobic respiration, nitrogen metabolism and glycoprotein biosynthesis. Cy-5 labeled genomic DNA from each evolvant (red bars) was hybridized against Cy-3 labeled genomic DNA from JA122 (green bars). The log2 ratio of hybridization intensities is depicted along a linear map of the E. coli K-12 MG1655 chromosome with genes closest to the origin at the top. Grey lines denote a 2-fold difference in target hybridization. The deleted portion of the CV103 chromosome shown as an excess of hybridization in the reference channel encompasses the 27 genes detailed in the table to the right.